2012
Hodapp, T; Sester, U; Mack, U; Singh, M; Meier, T; Wiech, E; Fisch, P; Ehl, S; Sester, M
Massive monoclonal expansion of CD4 T-cells specific for a Mycobacterium tuberculosis ESAT-6 peptide Artikel
In: The European respiratory journal, Bd. 40, Nr. 1, S. 152–160, 2012.
@article{Hodapp.2012,
title = {Massive monoclonal expansion of CD4 T-cells specific for a Mycobacterium tuberculosis ESAT-6 peptide},
author = {T Hodapp and U Sester and U Mack and M Singh and T Meier and E Wiech and P Fisch and S Ehl and M Sester},
doi = {10.1183/09031936.00175611},
year = {2012},
date = {2012-01-01},
urldate = {2012-01-01},
journal = {The European respiratory journal},
volume = {40},
number = {1},
pages = {152--160},
abstract = {T-cell responses towards tuberculin (purified protein derivative; PPD) or the Mycobacterium tuberculosis-specific antigens early secretory antigenic target (ESAT)-6 and culture filtrate protein-10 are indicative of prior contact with mycobacterial antigens. In this study, we investigated the exceptional case of a 75-yr-old patient who devoted more than one-third of his CD4 T-cells against PPD and ESAT-6. Antigen-specific T-cells were characterised using flow cytometric intracellular cytokine staining, ELISPOT assay, proliferation assays, and T-cell receptor spectratyping. T-cell frequencies were far above those found in age-matched controls (median 0.33%, range 0.05-6.32%) and remained at high levels for textgreater2 yrs. The patient initially presented with haemoptysis, but active tuberculosis was ruled out by repeated analysis of sputum and bronchoalveolar lavage fluid. Skin testing was negative and haemoptyses did not have a M. tuberculosis-related aetiology. Phenotypical and functional properties of specific T-cells were consistent with a terminally differentiated effector-memory phenotype with capacity to produce interferon-textgreekg, interleukin-2 and tumour necrosis factor-textgree\k{a}. Epitope mapping showed that the CD4 T-cells were directed against a single peptide from ESAT-6 (amino acid 5-20) that was presented in context of HLA-DR. T-cell receptor Vtextgreekb-spectratyping and sequencing of specific CD4 T-cells revealed a prominent peak fraction of monoclonal origin. In conclusion, similar to monoclonal gammopathies of undetermined significance, this may represent the first T-cell counterpart with known specificity against M. tuberculosis.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
T-cell responses towards tuberculin (purified protein derivative; PPD) or the Mycobacterium tuberculosis-specific antigens early secretory antigenic target (ESAT)-6 and culture filtrate protein-10 are indicative of prior contact with mycobacterial antigens. In this study, we investigated the exceptional case of a 75-yr-old patient who devoted more than one-third of his CD4 T-cells against PPD and ESAT-6. Antigen-specific T-cells were characterised using flow cytometric intracellular cytokine staining, ELISPOT assay, proliferation assays, and T-cell receptor spectratyping. T-cell frequencies were far above those found in age-matched controls (median 0.33%, range 0.05-6.32%) and remained at high levels for textgreater2 yrs. The patient initially presented with haemoptysis, but active tuberculosis was ruled out by repeated analysis of sputum and bronchoalveolar lavage fluid. Skin testing was negative and haemoptyses did not have a M. tuberculosis-related aetiology. Phenotypical and functional properties of specific T-cells were consistent with a terminally differentiated effector-memory phenotype with capacity to produce interferon-textgreekg, interleukin-2 and tumour necrosis factor-textgreeą. Epitope mapping showed that the CD4 T-cells were directed against a single peptide from ESAT-6 (amino acid 5-20) that was presented in context of HLA-DR. T-cell receptor Vtextgreekb-spectratyping and sequencing of specific CD4 T-cells revealed a prominent peak fraction of monoclonal origin. In conclusion, similar to monoclonal gammopathies of undetermined significance, this may represent the first T-cell counterpart with known specificity against M. tuberculosis.
Roth, B; Mohr, H; Enders, Martin; Garten, W; Gregersen, J P
In: Vaccine, Bd. 30, Nr. 3, S. 517–522, 2012, ISSN: 0264-410X.
@article{Roth.2012,
title = {Isolation of influenza viruses in MDCK 33016PF cells and clearance of contaminating respiratory viruses},
author = {B Roth and H Mohr and Martin Enders and W Garten and J P Gregersen},
doi = {10.1016/j.vaccine.2011.11.063},
issn = {0264-410X},
year = {2012},
date = {2012-01-01},
journal = {Vaccine},
volume = {30},
number = {3},
pages = {517--522},
abstract = {This paper summarizes results obtained by multiplex PCR screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in MDCK 33016PF cells. Using the ResPlexII v2.0 (Qiagen) multiplex PCR, 393 positive results were obtained in 468 clinical samples collected during an influenza season in Germany. The overall distribution of positive results was influenza A 42.0%, influenza B 38.7%, adenovirus 1.5%, bocavirus 0.5%, coronavirus 3.3%, enterovirus 5.6%, metapneumovirus 1.0%, parainfluenza virus 0.8%, rhinovirus 4.1%, and respiratory syncytial virus (RSV) 2.5%. Double infections of influenza virus together with another virus were found for adenovirus B and E, bocavirus, coronavirus, enterovirus and for rhinovirus. These other viruses were rapidly lost upon passages in MDCK 33016PF cells and under conditions as applied to influenza virus passaging. Clinical samples, in which no influenza virus but other viruses were found, were also subject to passages in MDCK 33016PF cells. Using lower inoculum dilutions than those normally applied for preparations containing influenza virus (total dilution of the original sample of approximately 10(4)), the positive results for the different viruses turned negative already after 2 or 3 passages in MDCK 33016PF cells. These results demonstrate that, under practical conditions as applied to grow influenza viruses, contaminating viruses can be effectively removed by passages in MDCK cells. In combination with their superior isolation efficiency, MDCK cells appear highly suitable to be used as an alternative to embryonated eggs to isolate and propagate influenza vaccine candidate viruses},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This paper summarizes results obtained by multiplex PCR screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in MDCK 33016PF cells. Using the ResPlexII v2.0 (Qiagen) multiplex PCR, 393 positive results were obtained in 468 clinical samples collected during an influenza season in Germany. The overall distribution of positive results was influenza A 42.0%, influenza B 38.7%, adenovirus 1.5%, bocavirus 0.5%, coronavirus 3.3%, enterovirus 5.6%, metapneumovirus 1.0%, parainfluenza virus 0.8%, rhinovirus 4.1%, and respiratory syncytial virus (RSV) 2.5%. Double infections of influenza virus together with another virus were found for adenovirus B and E, bocavirus, coronavirus, enterovirus and for rhinovirus. These other viruses were rapidly lost upon passages in MDCK 33016PF cells and under conditions as applied to influenza virus passaging. Clinical samples, in which no influenza virus but other viruses were found, were also subject to passages in MDCK 33016PF cells. Using lower inoculum dilutions than those normally applied for preparations containing influenza virus (total dilution of the original sample of approximately 10(4)), the positive results for the different viruses turned negative already after 2 or 3 passages in MDCK 33016PF cells. These results demonstrate that, under practical conditions as applied to grow influenza viruses, contaminating viruses can be effectively removed by passages in MDCK cells. In combination with their superior isolation efficiency, MDCK cells appear highly suitable to be used as an alternative to embryonated eggs to isolate and propagate influenza vaccine candidate viruses
Schmidt, T; Dirks, J; Enders, Martin; Gärtner, B C; Uhlmann-Schiffler, H; Sester, U; Sester, M
In: European Journal of immunology, Bd. 42, Nr. 7, S. 1755–1766, 2012, ISSN: 0014-2980.
@article{Schmidt.2012,
title = {CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection},
author = {T Schmidt and J Dirks and Martin Enders and B C G\"{a}rtner and H Uhlmann-Schiffler and U Sester and M Sester},
doi = {10.1002/eji.201242393},
issn = {0014-2980},
year = {2012},
date = {2012-01-01},
journal = {European Journal of immunology},
volume = {42},
number = {7},
pages = {1755--1766},
abstract = {Antigen-specific antibodies are well characterized after vaccination with pandemic H1N1 or seasonal influenza vaccines. However, knowledge on cellular immunity toward pandemic H1N1 after vaccination and infection and cross-reactivities toward seasonal antigens is limited. Nineteen individuals were vaccinated with the pandemic H1N1 vaccine. Among those, ten had been prevaccinated against seasonal influenza. CD4(+) T cells specific for pandemic H1N1 and for seasonal vaccine, and antibodies were monitored using flow cytometry and ELISA/neutralization assays, respectively. In addition, seven patients with acute pandemic influenza infection were analyzed. Pandemic H1N1 vaccination induced a strong 4.63-fold (IQR 4.16) increase in antigen-specific CD4(+) T cells that was more pronounced in individuals not prevaccinated with seasonal influenza (p = 0.01). T-cell levels toward seasonal vaccine concomitantly rose by 2.71-fold (IQR 2.26). Likewise, prevaccination with seasonal influenza induced a less pronounced increase in specific antibodies. Influenza-specific T cells in vaccinees had a Th1 phenotype mainly coexpressing IFN-gamma and IL-2, whereas patients with active pandemic influenza showed a shift toward cells predominantly expressing IFN-gamma. In conclusion, T cells toward seasonal influenza antigens cross-react with pandemic H1N1 antigens and affect induction of specific T cells after pandemic influenza vaccination. In addition, the cytokine patterns of specific T cells during acute H1N1 infection and after vaccination differ, and the predominantly dual-positive cytokine profile of vaccine-induced T cells suggests sufficient functionality to confer successful virus control},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Antigen-specific antibodies are well characterized after vaccination with pandemic H1N1 or seasonal influenza vaccines. However, knowledge on cellular immunity toward pandemic H1N1 after vaccination and infection and cross-reactivities toward seasonal antigens is limited. Nineteen individuals were vaccinated with the pandemic H1N1 vaccine. Among those, ten had been prevaccinated against seasonal influenza. CD4(+) T cells specific for pandemic H1N1 and for seasonal vaccine, and antibodies were monitored using flow cytometry and ELISA/neutralization assays, respectively. In addition, seven patients with acute pandemic influenza infection were analyzed. Pandemic H1N1 vaccination induced a strong 4.63-fold (IQR 4.16) increase in antigen-specific CD4(+) T cells that was more pronounced in individuals not prevaccinated with seasonal influenza (p = 0.01). T-cell levels toward seasonal vaccine concomitantly rose by 2.71-fold (IQR 2.26). Likewise, prevaccination with seasonal influenza induced a less pronounced increase in specific antibodies. Influenza-specific T cells in vaccinees had a Th1 phenotype mainly coexpressing IFN-gamma and IL-2, whereas patients with active pandemic influenza showed a shift toward cells predominantly expressing IFN-gamma. In conclusion, T cells toward seasonal influenza antigens cross-react with pandemic H1N1 antigens and affect induction of specific T cells after pandemic influenza vaccination. In addition, the cytokine patterns of specific T cells during acute H1N1 infection and after vaccination differ, and the predominantly dual-positive cytokine profile of vaccine-induced T cells suggests sufficient functionality to confer successful virus control
Eggers, M; Roth, B; Schweiger, B; Schmid, M; Gregersen, J P; Enders, M
In: European journal of clinical microbiology & infectious diseases, Bd. 31, Nr. 6, S. 1257–1265, 2012, ISSN: 0934-9723.
@article{Eggers.2012,
title = {Comparison of the novel ResPlex III assay and existing techniques for the detection and subtyping of influenza virus during the influenza season 2006-2007},
author = {M Eggers and B Roth and B Schweiger and M Schmid and J P Gregersen and M Enders},
doi = {10.1007/s10096-011-1437-1},
issn = {0934-9723},
year = {2012},
date = {2012-01-01},
journal = {European journal of clinical microbiology \& infectious diseases},
volume = {31},
number = {6},
pages = {1257--1265},
abstract = {Influenza virus is a major cause of disease worldwide. The accurate detection and further subtyping of influenza A viruses are important for epidemiologic surveillance, and subsequent comprehensive characterization of circulating influenza viruses is essential for the selection of an optimal vaccine composition. ResPlex III is a new multiplex reverse transcriptase polymerase chain reaction (RT-PCR)-based method for detecting, typing, and subtyping influenza virus in clinical specimens. The ResPlex III assay was compared with other methods with respect to sensitivity and accuracy, using 450 clinical specimens obtained from subjects throughout Germany during the 2006-2007 influenza season. Samples were analyzed for the presence of influenza virus in Madin-Darby canine kidney (MDCK) cells by rapid cell culture using peroxidase staining and conventional cell culture confirmed by hemagglutination inhibition assay, a rapid diagnostic assay (Directigen Flu A+B test; BD Diagnostic Systems, Heidelberg, Germany), in-house real-time RT-PCR (RRT-PCR), and ResPlex III (Qiagen, Hilden, Germany). ResPlex III had the highest sensitivity for detecting influenza virus in clinical specimens, followed by in-house RRT-PCR (96% compared with ResPlex III). Conventional cell culture in MDCK cells, rapid culture, and quick test assays were substantially less sensitive (55%, 72%, and 39%, respectively). Virus subtyping results were identical using ResPlex III and the standard virological subtyping method, hemagglutination inhibition. ResPlex III is a quick, accurate, and sensitive assay for detecting and typing influenza A and B viruses and subtyping influenza A viruses in clinical specimens, and might be considered for a supplemental role in worldwide seasonal and pandemic influenza surveillance},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Influenza virus is a major cause of disease worldwide. The accurate detection and further subtyping of influenza A viruses are important for epidemiologic surveillance, and subsequent comprehensive characterization of circulating influenza viruses is essential for the selection of an optimal vaccine composition. ResPlex III is a new multiplex reverse transcriptase polymerase chain reaction (RT-PCR)-based method for detecting, typing, and subtyping influenza virus in clinical specimens. The ResPlex III assay was compared with other methods with respect to sensitivity and accuracy, using 450 clinical specimens obtained from subjects throughout Germany during the 2006-2007 influenza season. Samples were analyzed for the presence of influenza virus in Madin-Darby canine kidney (MDCK) cells by rapid cell culture using peroxidase staining and conventional cell culture confirmed by hemagglutination inhibition assay, a rapid diagnostic assay (Directigen Flu A+B test; BD Diagnostic Systems, Heidelberg, Germany), in-house real-time RT-PCR (RRT-PCR), and ResPlex III (Qiagen, Hilden, Germany). ResPlex III had the highest sensitivity for detecting influenza virus in clinical specimens, followed by in-house RRT-PCR (96% compared with ResPlex III). Conventional cell culture in MDCK cells, rapid culture, and quick test assays were substantially less sensitive (55%, 72%, and 39%, respectively). Virus subtyping results were identical using ResPlex III and the standard virological subtyping method, hemagglutination inhibition. ResPlex III is a quick, accurate, and sensitive assay for detecting and typing influenza A and B viruses and subtyping influenza A viruses in clinical specimens, and might be considered for a supplemental role in worldwide seasonal and pandemic influenza surveillance
Ettinger, J; Hofmann, J; Enders, Martin; Tewald, Friedemann; Oehme, R M; Rosenfeld, U M; Ali, H S; Schlegel, M; Essbauer, S; Osterberg, A; Jacob, J; Reil, D; Klempa, B; Ulrich, R G; Kruger, D H
Multiple synchronous outbreaks of Puumala virus, Germany, 2010 Artikel
In: Emerging Infectious Diseases, Bd. 18, Nr. 9, S. 1461–1464, 2012, ISSN: 1080-6040.
@article{Ettinger.2012,
title = {Multiple synchronous outbreaks of Puumala virus, Germany, 2010},
author = {J Ettinger and J Hofmann and Martin Enders and Friedemann Tewald and R M Oehme and U M Rosenfeld and H S Ali and M Schlegel and S Essbauer and A Osterberg and J Jacob and D Reil and B Klempa and R G Ulrich and D H Kruger},
doi = {10.3201/eid1809.111447},
issn = {1080-6040},
year = {2012},
date = {2012-01-01},
journal = {Emerging Infectious Diseases},
volume = {18},
number = {9},
pages = {1461--1464},
abstract = {To investigate 2,017 cases of hantavirus disease in Germany, we compared 38 new patient-derived Puumala virus RNA sequences identified in 2010 with bank vole-derived small segment RNA sequences. The epidemic process was driven by outbreaks of 6 Puumala virus clades comprising strains of human and vole origin. Each clade corresponded to a different outbreak region},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
To investigate 2,017 cases of hantavirus disease in Germany, we compared 38 new patient-derived Puumala virus RNA sequences identified in 2010 with bank vole-derived small segment RNA sequences. The epidemic process was driven by outbreaks of 6 Puumala virus clades comprising strains of human and vole origin. Each clade corresponded to a different outbreak region
Hofmann, J; Führer, A; Bolz, M; Waldschlager-Terpe, J; Meier, M; Ludders, D; Enders, Martin; Oltmann, A; Meisel, H; Kruger, D H
Hantavirus infections by Puumala or Dobrava-Belgrade virus in pregnant women Artikel
In: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Bd. 55, Nr. 3, S. 266–269, 2012, ISSN: 1386-6532.
@article{Hofmann.2012,
title = {Hantavirus infections by Puumala or Dobrava-Belgrade virus in pregnant women},
author = {J Hofmann and A F\"{u}hrer and M Bolz and J Waldschlager-Terpe and M Meier and D Ludders and Martin Enders and A Oltmann and H Meisel and D H Kruger},
doi = {10.1016/j.jcv.2012.07.011},
issn = {1386-6532},
year = {2012},
date = {2012-01-01},
journal = {Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
volume = {55},
number = {3},
pages = {266--269},
abstract = {BACKGROUND: Hantavirus infection in humans usually occurs via inhalation of infectious aerosolized excreta of rodents. Horizontal human-to-human transmission was reported only for the highly virulent Andes virus. The likelihood of vertical transmission and the clinical outcome of hantavirus infections in pregnancy is still unpredictable. OBJECTIVES: Very few data were published about the impact of hantaviruses in pregnancy. Here we present four cases of pregnant women infected by European hantaviruses. The risk of vertical virus transmission was investigated. STUDY DESIGN: Four pregnant women with clinical signs of acute hantavirus disease were investigated for hantavirus IgM and IgG after onset of clinical symptoms. Furthermore, the newborns were tested for presence of viral RNA and antibodies in cord blood and, if any parameter was found positive, 8-12 months after delivery. RESULTS: Four women suffered from a hantavirus infection, two of them due to infection by Puumala virus and two by Dobrava-Belgrade virus. Three women delivered healthy babies by vaginal route and one woman by Caesarean section (week 28). In no case hantavirus RNA was detected in cord blood after delivery or in the 8-12 month old babies. Hantavirus IgG was detectable in the cord blood of 3 babies (but not in the preterm child); these antibodies disappeared after 8-12 months indicating a passive transfer of immunoglobulins. No child had any clinical sign of hantavirus infection. CONCLUSIONS: In this study, the absence of vertical hantavirus transmission was demonstrated for pregnant women with onset of hantavirus disease between gestation weeks 14 and 28},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Hantavirus infection in humans usually occurs via inhalation of infectious aerosolized excreta of rodents. Horizontal human-to-human transmission was reported only for the highly virulent Andes virus. The likelihood of vertical transmission and the clinical outcome of hantavirus infections in pregnancy is still unpredictable. OBJECTIVES: Very few data were published about the impact of hantaviruses in pregnancy. Here we present four cases of pregnant women infected by European hantaviruses. The risk of vertical virus transmission was investigated. STUDY DESIGN: Four pregnant women with clinical signs of acute hantavirus disease were investigated for hantavirus IgM and IgG after onset of clinical symptoms. Furthermore, the newborns were tested for presence of viral RNA and antibodies in cord blood and, if any parameter was found positive, 8-12 months after delivery. RESULTS: Four women suffered from a hantavirus infection, two of them due to infection by Puumala virus and two by Dobrava-Belgrade virus. Three women delivered healthy babies by vaginal route and one woman by Caesarean section (week 28). In no case hantavirus RNA was detected in cord blood after delivery or in the 8-12 month old babies. Hantavirus IgG was detectable in the cord blood of 3 babies (but not in the preterm child); these antibodies disappeared after 8-12 months indicating a passive transfer of immunoglobulins. No child had any clinical sign of hantavirus infection. CONCLUSIONS: In this study, the absence of vertical hantavirus transmission was demonstrated for pregnant women with onset of hantavirus disease between gestation weeks 14 and 28
2011
Enders, M; Enders, G
Pränatale Infektionen - Übertragungswege, Komplikationen, Therapie Buch
Hans Marseille Verlag GmbH, München, 2011, ISBN: 978-3-88616-141-6.
@book{Enders.2011,
title = {Pr\"{a}natale Infektionen - \"{U}bertragungswege, Komplikationen, Therapie},
author = {M Enders and G Enders},
isbn = {978-3-88616-141-6},
year = {2011},
date = {2011-01-01},
publisher = {Hans Marseille Verlag GmbH},
address = {M\"{u}nchen},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
Enders, M
Fetale Virusinfektionen Artikel
In: Der Gynäkologe, Bd. 44, Nr. 5, S. 385–400, 2011, ISSN: 0017-5994.
@article{Enders.2011b,
title = {Fetale Virusinfektionen},
author = {M Enders},
doi = {10.1007/s00129-011-2761-3},
issn = {0017-5994},
year = {2011},
date = {2011-01-01},
journal = {Der Gyn\"{a}kologe},
volume = {44},
number = {5},
pages = {385--400},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Handrick, W; Enders, Martin; Borte, M
Konnatale Syphilis - es gibt sie noch! Artikel
In: pädiatrische praxis, Bd. 76, Nr. 3, S. 445–450, 2011.
@article{Handrick.2011,
title = {Konnatale Syphilis - es gibt sie noch!},
author = {W Handrick and Martin Enders and M Borte},
year = {2011},
date = {2011-01-01},
journal = {p\"{a}diatrische praxis},
volume = {76},
number = {3},
pages = {445--450},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Kagan, Karl Oliver; Mylonas, Ioannis; Enders, Martin; Wallwiener, D; Friese, K; Jahn, G; Hamprecht, K
Intrauterine Zytomegalievirusinfektion Artikel
In: Der Gynäkologe, Bd. 44, Nr. 8, S. 601–609, 2011, ISSN: 0017-5994.
@article{Kagan.2011,
title = {Intrauterine Zytomegalievirusinfektion},
author = {Karl Oliver Kagan and Ioannis Mylonas and Martin Enders and D Wallwiener and K Friese and G Jahn and K Hamprecht},
doi = {10.1007/s00129-011-2776-9},
issn = {0017-5994},
year = {2011},
date = {2011-01-01},
journal = {Der Gyn\"{a}kologe},
volume = {44},
number = {8},
pages = {601--609},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Enders, G; Daiminger, A; Bäder, U; Exler, S; Enders, M
In: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Bd. 52, Nr. 3, S. 244–246, 2011.
@article{Enders.2011c,
title = {Intrauterine transmission and clinical outcome of 248 pregnancies with primary cytomegalovirus infection in relation to gestational age},
author = {G Enders and A Daiminger and U B\"{a}der and S Exler and M Enders},
doi = {10.1016/j.jcv.2011.07.005},
year = {2011},
date = {2011-01-01},
urldate = {2011-01-01},
journal = {Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
volume = {52},
number = {3},
pages = {244--246},
abstract = {BACKGROUND: The risk of intrauterine cytomegalovirus (CMV) infection and disease in the fetus or newborn largely depends on time of primary maternal infection during pregnancy. OBJECTIVES: Prospective cohort study of pregnancy outcome in relation to gestational age at primary maternal CMV infection. STUDY DESIGN: In a total of 248 pregnancies with primary infection the onset of infection was determined by IgG seroconversion, IgG avidity and/or onset of clinical symptoms. Congenital infection was diagnosed by CMV detection in amniotic fluid, fetal tissue or urine of the neonate in the first 2 weeks of life. Clinical symptoms were retrieved from ultrasound and medical records. RESULTS: The intrauterine transmission rates following primary CMV infection in the pre- and periconceptional period were 16.7% (4/24) and 34.5% (10/29), respectively. For the first, second and third trimester of pregnancy transmission rates were 30.1% (25/83), 38.2% (29/76) and 72.2% (26/36), respectively. The rate of symptomatically infected fetuses or newborns at birth was 22.8% for any symptoms and 10.3% for severe manifestations. No symptoms were observed in infected newborns of mothers with primary infection in the preconceptional period and in the third trimester. CONCLUSIONS: The risk of intrauterine transmission following primary maternal infection in the third trimester is high, but the risk of neonatal disease is low. The highest risk of severe symptoms in the fetus and newborn exists around conception and in the first trimester of pregnancy},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: The risk of intrauterine cytomegalovirus (CMV) infection and disease in the fetus or newborn largely depends on time of primary maternal infection during pregnancy. OBJECTIVES: Prospective cohort study of pregnancy outcome in relation to gestational age at primary maternal CMV infection. STUDY DESIGN: In a total of 248 pregnancies with primary infection the onset of infection was determined by IgG seroconversion, IgG avidity and/or onset of clinical symptoms. Congenital infection was diagnosed by CMV detection in amniotic fluid, fetal tissue or urine of the neonate in the first 2 weeks of life. Clinical symptoms were retrieved from ultrasound and medical records. RESULTS: The intrauterine transmission rates following primary CMV infection in the pre- and periconceptional period were 16.7% (4/24) and 34.5% (10/29), respectively. For the first, second and third trimester of pregnancy transmission rates were 30.1% (25/83), 38.2% (29/76) and 72.2% (26/36), respectively. The rate of symptomatically infected fetuses or newborns at birth was 22.8% for any symptoms and 10.3% for severe manifestations. No symptoms were observed in infected newborns of mothers with primary infection in the preconceptional period and in the third trimester. CONCLUSIONS: The risk of intrauterine transmission following primary maternal infection in the third trimester is high, but the risk of neonatal disease is low. The highest risk of severe symptoms in the fetus and newborn exists around conception and in the first trimester of pregnancy
Terletskaia-Ladwig, Elena; Eggers, Maren; Enders, Martin; Regnath, T
[Epidemiological aspects of gastrointestinal infections] Artikel
In: Deutsche Medizinische Wochenschrift, Bd. 136, Nr. 3, S. 69–75, 2011, ISSN: 0012-0472.
@article{TerletskaiaLadwig.2011,
title = {[Epidemiological aspects of gastrointestinal infections]},
author = {Elena Terletskaia-Ladwig and Maren Eggers and Martin Enders and T Regnath},
issn = {0012-0472},
year = {2011},
date = {2011-01-01},
journal = {Deutsche Medizinische Wochenschrift},
volume = {136},
number = {3},
pages = {69--75},
abstract = {BACKGROUND AND OBJECTIVES: The aim of this study was to investigate seasonal patterns and age-associated trends of the main bacterial, viral, and parasitic enteric pathogens in Southwest Germany. PATIENTS AND METHODS: From January 2002 through December 2008 a total of 99,057 patients were tested for Norovirus, Rotavirus, bacterial pathogens, Cryptosporidium parvum (C. parvum), and Giardia lamblia (G. lamblia). RESULTS: All these pathogens were detected throughout the whole year. But there were distinctive seasonal patterns of activity of the following pathogens being detected: norovirus was detected mainly from September through April. The highest rotovirus activity was observed from December through June. But bacterial pathogens und C. parvum were found mainly from June to November. The percentage of positive results during the months with the highest activity was 10 - 49% for norovirus, 25% - 41% for rotavirus, 14 - 18% for bacterial infection and 3 - 4 % for C. parvum. G. lamblia and adenovirus were found throughout the year in 7 - 15% and 3 - 10% of samples, respectively. Moreover, the detection rate of different pathogens depended on patient age. In infants younger than one year, rotavirus, norovirus and adenovirus were most frequently isolated pathogenes. Stool samples from kindergarden- and school-age children were positive largely for bacterial pathogens such as Salmonella and Campylobacter particularly in late summer or early autum. In patients older than 60 years, norovirus, rotavirus, and toxin producing Clostridium difficile strains were the most common pathogens. CONCLUSIONS: In view of the age and season related frequency of detection of enteric pathogens, a step-by-step diagnosis of gastrointestinal tract infections is recommended. Considering that most pathogens are detected sporadically over the whole year, the analysis of negative samples should be appropriately expanded. The knowledge of seasonal occurrence can also be applied to improve the application of hygienic measures},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND AND OBJECTIVES: The aim of this study was to investigate seasonal patterns and age-associated trends of the main bacterial, viral, and parasitic enteric pathogens in Southwest Germany. PATIENTS AND METHODS: From January 2002 through December 2008 a total of 99,057 patients were tested for Norovirus, Rotavirus, bacterial pathogens, Cryptosporidium parvum (C. parvum), and Giardia lamblia (G. lamblia). RESULTS: All these pathogens were detected throughout the whole year. But there were distinctive seasonal patterns of activity of the following pathogens being detected: norovirus was detected mainly from September through April. The highest rotovirus activity was observed from December through June. But bacterial pathogens und C. parvum were found mainly from June to November. The percentage of positive results during the months with the highest activity was 10 - 49% for norovirus, 25% - 41% for rotavirus, 14 - 18% for bacterial infection and 3 - 4 % for C. parvum. G. lamblia and adenovirus were found throughout the year in 7 - 15% and 3 - 10% of samples, respectively. Moreover, the detection rate of different pathogens depended on patient age. In infants younger than one year, rotavirus, norovirus and adenovirus were most frequently isolated pathogenes. Stool samples from kindergarden- and school-age children were positive largely for bacterial pathogens such as Salmonella and Campylobacter particularly in late summer or early autum. In patients older than 60 years, norovirus, rotavirus, and toxin producing Clostridium difficile strains were the most common pathogens. CONCLUSIONS: In view of the age and season related frequency of detection of enteric pathogens, a step-by-step diagnosis of gastrointestinal tract infections is recommended. Considering that most pathogens are detected sporadically over the whole year, the analysis of negative samples should be appropriately expanded. The knowledge of seasonal occurrence can also be applied to improve the application of hygienic measures
Aperlic, K; Tietz, H-J; Erhard, M; Regnath, T
25-jähriger Patient mit Hornhautinfiltrat Artikel
In: Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft, Bd. 108, Nr. 5, S. 463–466, 2011.
@article{Aperlic.2011,
title = {25-j\"{a}hriger Patient mit Hornhautinfiltrat},
author = {K Aperlic and H-J Tietz and M Erhard and T Regnath},
doi = {10.1007/s00347-010-2317-2},
year = {2011},
date = {2011-01-01},
journal = {Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft},
volume = {108},
number = {5},
pages = {463--466},
abstract = {Filamentous fungal keratitis represents a serious infection of the eye. When corneal infiltrates appear, particularly in those who wear contact lenses, mycological assessment should already be performed initially so that filamentous fungal keratitis can be recognized early and treated. Keratitis caused by Fusarium responds well in most cases to topical therapy with ketoconazole or other antimycotic agents so that surgical intervention is only necessary in advanced or treatment-refractory cases.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Filamentous fungal keratitis represents a serious infection of the eye. When corneal infiltrates appear, particularly in those who wear contact lenses, mycological assessment should already be performed initially so that filamentous fungal keratitis can be recognized early and treated. Keratitis caused by Fusarium responds well in most cases to topical therapy with ketoconazole or other antimycotic agents so that surgical intervention is only necessary in advanced or treatment-refractory cases.
Hospach, Toni; Langendörfer, M; Kalle, T V; Tewald, Friedemann; Wirth, T; Dannecker, G E
Mimicry of lyme arthritis by synovial hemangioma Artikel
In: Rheumatology international, Bd. 31, Nr. 12, S. 1639–1643, 2011.
@article{Hospach.2011,
title = {Mimicry of lyme arthritis by synovial hemangioma},
author = {Toni Hospach and M Langend\"{o}rfer and T V Kalle and Friedemann Tewald and T Wirth and G E Dannecker},
doi = {10.1007/s00296-009-1320-x},
year = {2011},
date = {2011-01-01},
journal = {Rheumatology international},
volume = {31},
number = {12},
pages = {1639--1643},
abstract = {To report on the differential diagnosis of lyme arthritis and synovial hemangioma due to similar clinical and radiological signs and symptoms. A 15-year-old boy presented at the age of 9 with recurrent rather painless swelling of the right knee. Altogether four episodes lasting for 1-2 weeks each occurred over a period of 18 months before medical advice was sought. Physical examination revealed only a slightly limited range of motion. Living in an endemic area of borreliosis, he reported a tick bite 6 months prior to onset of his symptoms with erythema migrans and was treated for 10 days with amoxicillin. Serology revealed two positive unspecific bands in IgG immunoblot (p41 and 66) with slight positivity for ELISA. Ultrasound revealed synovial thickening and increased fluid. Despite the weak positive serology a diagnosis of lyme arthritis could not be excluded and intravenous antibiotic treatment with ceftriaxone was started. After two further relapses antiinflammatory therapy including intraarticular steroids were introduced with no long lasting effect. A chronical disease developed with alternate periods of swelling and almost complete remission. Ultrasound as well as MRI demonstrated ongoing signs of synovitis, therefore after further progression, a diagnostic arthroscopy was performed showing an inconspicuous knee joint. A second MRI showed focal suprapatellar enhancement and was followed by open arthrotomy revealing a histopathological proven synovial cavernous juxtaarticular hemangioma. To our knowledge, the differential diagnosis of lyme arthritis and synovial hemangioma has not yet been reported despite obvious clinical similarities. In conclusion, in children and adolescents synovial hemangioma has to be considered in differential diagnosis of recurrent knee swelling. Early diagnosis is important to prevent prolonged suffering from chronic joint swelling with probable joint damages, unnecessary treatment procedures and as well school and sports absenteeism.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
To report on the differential diagnosis of lyme arthritis and synovial hemangioma due to similar clinical and radiological signs and symptoms. A 15-year-old boy presented at the age of 9 with recurrent rather painless swelling of the right knee. Altogether four episodes lasting for 1-2 weeks each occurred over a period of 18 months before medical advice was sought. Physical examination revealed only a slightly limited range of motion. Living in an endemic area of borreliosis, he reported a tick bite 6 months prior to onset of his symptoms with erythema migrans and was treated for 10 days with amoxicillin. Serology revealed two positive unspecific bands in IgG immunoblot (p41 and 66) with slight positivity for ELISA. Ultrasound revealed synovial thickening and increased fluid. Despite the weak positive serology a diagnosis of lyme arthritis could not be excluded and intravenous antibiotic treatment with ceftriaxone was started. After two further relapses antiinflammatory therapy including intraarticular steroids were introduced with no long lasting effect. A chronical disease developed with alternate periods of swelling and almost complete remission. Ultrasound as well as MRI demonstrated ongoing signs of synovitis, therefore after further progression, a diagnostic arthroscopy was performed showing an inconspicuous knee joint. A second MRI showed focal suprapatellar enhancement and was followed by open arthrotomy revealing a histopathological proven synovial cavernous juxtaarticular hemangioma. To our knowledge, the differential diagnosis of lyme arthritis and synovial hemangioma has not yet been reported despite obvious clinical similarities. In conclusion, in children and adolescents synovial hemangioma has to be considered in differential diagnosis of recurrent knee swelling. Early diagnosis is important to prevent prolonged suffering from chronic joint swelling with probable joint damages, unnecessary treatment procedures and as well school and sports absenteeism.
Lüthgens, Kai; Abele, H; Alkier, R; Hoopmann, M; Kagan, Karl Oliver
In: Ultraschall in der Medizin (Stuttgart, Germany : 1980), Bd. 32, Nr. 4, S. 367–372, 2011.
@article{Luthgens.2011,
title = {Cross-Validierung des Screening-Algorithmus der FMF London an 38,700 Ersttrimester-Untersuchungen in Deutschland},
author = {Kai L\"{u}thgens and H Abele and R Alkier and M Hoopmann and Karl Oliver Kagan},
doi = {10.1055/s-0031-1273348},
year = {2011},
date = {2011-01-01},
journal = {Ultraschall in der Medizin (Stuttgart, Germany : 1980)},
volume = {32},
number = {4},
pages = {367--372},
abstract = {PURPOSE
Validation of the performance of the new algorithm of the FMF London for screening for trisomy 21 using a combination of maternal age, fetal nuchal translucency (NT) and maternal serum free textgreekb-hCG and PAPP-A.
MATERIALS AND METHODS
Between 2002 and 2007, NT was measured prospectively in 39,004 pregnancies in the context of routinely performed first trimester screening in Germany. Individual trisomy 21 risks were calculated by a combination of NT, maternal age, free textgreekb-hCG, and PAPP-A using the FMF algorithm in force at the time of investigation. In this study we recalculated the trisomy 21 risks applying the new algorithm of the FMF UK that includes the new mixture model for the NT measurement.
RESULTS
38,751 singleton pregnancies could be included in the study of which 109 (0.3 %) had a trisomy 21. Only 35 % of the NT measurements of euploids were above the median and 25 % of the NT measurements were below the 5th percentile of the FMF UK. For sonographers that were qualified according to level II or III of the German DEGUM system, the median NT of fetuses with trisomy 21 was 0.9 mm above the median of the FMF UK and only 0.5 mm above the median for all other sonographers. Despite the limited performance of the NT measurement, the overall detection rate for a trisomy 21 was 90.8 % when combining the NT with maternal age, PAPP-A and free textgreekb-hCG. The overall false-positive rate for a trisomy 21 was 6.5 % at a cut-off value of 1:300.
CONCLUSION
In this study we were able to show that the use of the new risk algorithm of the FMF UK leads to a trisomy 21 detection rate of about 90 % at a 5 % false-positive rate in a German collective despite a significant underestimation of the NT.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
PURPOSE
Validation of the performance of the new algorithm of the FMF London for screening for trisomy 21 using a combination of maternal age, fetal nuchal translucency (NT) and maternal serum free textgreekb-hCG and PAPP-A.
MATERIALS AND METHODS
Between 2002 and 2007, NT was measured prospectively in 39,004 pregnancies in the context of routinely performed first trimester screening in Germany. Individual trisomy 21 risks were calculated by a combination of NT, maternal age, free textgreekb-hCG, and PAPP-A using the FMF algorithm in force at the time of investigation. In this study we recalculated the trisomy 21 risks applying the new algorithm of the FMF UK that includes the new mixture model for the NT measurement.
RESULTS
38,751 singleton pregnancies could be included in the study of which 109 (0.3 %) had a trisomy 21. Only 35 % of the NT measurements of euploids were above the median and 25 % of the NT measurements were below the 5th percentile of the FMF UK. For sonographers that were qualified according to level II or III of the German DEGUM system, the median NT of fetuses with trisomy 21 was 0.9 mm above the median of the FMF UK and only 0.5 mm above the median for all other sonographers. Despite the limited performance of the NT measurement, the overall detection rate for a trisomy 21 was 90.8 % when combining the NT with maternal age, PAPP-A and free textgreekb-hCG. The overall false-positive rate for a trisomy 21 was 6.5 % at a cut-off value of 1:300.
CONCLUSION
In this study we were able to show that the use of the new risk algorithm of the FMF UK leads to a trisomy 21 detection rate of about 90 % at a 5 % false-positive rate in a German collective despite a significant underestimation of the NT.
Validation of the performance of the new algorithm of the FMF London for screening for trisomy 21 using a combination of maternal age, fetal nuchal translucency (NT) and maternal serum free textgreekb-hCG and PAPP-A.
MATERIALS AND METHODS
Between 2002 and 2007, NT was measured prospectively in 39,004 pregnancies in the context of routinely performed first trimester screening in Germany. Individual trisomy 21 risks were calculated by a combination of NT, maternal age, free textgreekb-hCG, and PAPP-A using the FMF algorithm in force at the time of investigation. In this study we recalculated the trisomy 21 risks applying the new algorithm of the FMF UK that includes the new mixture model for the NT measurement.
RESULTS
38,751 singleton pregnancies could be included in the study of which 109 (0.3 %) had a trisomy 21. Only 35 % of the NT measurements of euploids were above the median and 25 % of the NT measurements were below the 5th percentile of the FMF UK. For sonographers that were qualified according to level II or III of the German DEGUM system, the median NT of fetuses with trisomy 21 was 0.9 mm above the median of the FMF UK and only 0.5 mm above the median for all other sonographers. Despite the limited performance of the NT measurement, the overall detection rate for a trisomy 21 was 90.8 % when combining the NT with maternal age, PAPP-A and free textgreekb-hCG. The overall false-positive rate for a trisomy 21 was 6.5 % at a cut-off value of 1:300.
CONCLUSION
In this study we were able to show that the use of the new risk algorithm of the FMF UK leads to a trisomy 21 detection rate of about 90 % at a 5 % false-positive rate in a German collective despite a significant underestimation of the NT.
Schalasta, Gunnar
Test auf Routinetauglichkeit bestanden (cobas 4800) Artikel
In: Diagnostik im Dialog, Bd. 31, Nr. 1, 2011.
@article{Schalasta.2011,
title = {Test auf Routinetauglichkeit bestanden (cobas 4800)},
author = {Gunnar Schalasta},
year = {2011},
date = {2011-01-01},
journal = {Diagnostik im Dialog},
volume = {31},
number = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Tewald, Friedemann
Borrelieninfektion: Diagnostische Fallstricke Artikel
In: Der Allgemeinarzt, Bd. 33, Nr. 19, S. 14, 2011, ISSN: 0172-7249.
@article{Tewald.2011,
title = {Borrelieninfektion: Diagnostische Fallstricke},
author = {Friedemann Tewald},
url = {http://www.zbmed.de/ccmedimages/2011/ZBMED-201111245152-1.pdf},
issn = {0172-7249},
year = {2011},
date = {2011-01-01},
journal = {Der Allgemeinarzt},
volume = {33},
number = {19},
pages = {14},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Abele, H; Lüthgens, Kai; Hoopmann, M; Kagan, Karl Oliver
Impact of the maternal age-related risk in first-trimester combined screening for trisomy 21 Artikel
In: Fetal diagnosis and therapy, Bd. 30, Nr. 2, S. 135–140, 2011, ISSN: 1015-3837.
@article{Abele.2011,
title = {Impact of the maternal age-related risk in first-trimester combined screening for trisomy 21},
author = {H Abele and Kai L\"{u}thgens and M Hoopmann and Karl Oliver Kagan},
doi = {10.1159/000327157},
issn = {1015-3837},
year = {2011},
date = {2011-01-01},
journal = {Fetal diagnosis and therapy},
volume = {30},
number = {2},
pages = {135--140},
abstract = {OBJECTIVE
To examine the impact of the maternal age-related risk in first-trimester combined screening for trisomy 21.
METHODS
Prospective assessment of risk for trisomy 21 by a combination of maternal age, fetal NT thickness and maternal serum PAPP-A and free textgreekb-hCG at 11+0 to 13+6 weeks of gestation between April 2002 and February 2007. Screening for trisomy 21 by patient-specific risks based on the maternal and gestational age-related risk multiplied by a likelihood ratio for NT and for maternal serum biochemistry were compared with a screening policy that is only based on the combined likelihood ratio for fetal NT and maternal serum biochemistry.
RESULTS
The study population consisted of 38,603 euploid pregnancies and 109 fetuses with trisomy 21. In screening for trisomy 21 by fetal NT and maternal serum biochemistry in combination with and without maternal age with a fixed false-positive rate of 3%, the detection rate was 82.6 and 79.8%, respectively. In the group of women with a maternal age of less than 30 years and between 30 and 35 years, there was no difference in the detection rate. For women with a maternal age of 35 years or older, the detection rate increased from 77.1% without maternal age to 94.3% with maternal age, respectively.
CONCLUSION
The overall difference between first-trimester screening based on fetal NT and maternal serum biochemistry with and without maternal age is about 3%. In screening with a fixed cut-off, the maternal age-related risk keeps the false-positive rate low in younger women and increases the detection rate in older women.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
OBJECTIVE
To examine the impact of the maternal age-related risk in first-trimester combined screening for trisomy 21.
METHODS
Prospective assessment of risk for trisomy 21 by a combination of maternal age, fetal NT thickness and maternal serum PAPP-A and free textgreekb-hCG at 11+0 to 13+6 weeks of gestation between April 2002 and February 2007. Screening for trisomy 21 by patient-specific risks based on the maternal and gestational age-related risk multiplied by a likelihood ratio for NT and for maternal serum biochemistry were compared with a screening policy that is only based on the combined likelihood ratio for fetal NT and maternal serum biochemistry.
RESULTS
The study population consisted of 38,603 euploid pregnancies and 109 fetuses with trisomy 21. In screening for trisomy 21 by fetal NT and maternal serum biochemistry in combination with and without maternal age with a fixed false-positive rate of 3%, the detection rate was 82.6 and 79.8%, respectively. In the group of women with a maternal age of less than 30 years and between 30 and 35 years, there was no difference in the detection rate. For women with a maternal age of 35 years or older, the detection rate increased from 77.1% without maternal age to 94.3% with maternal age, respectively.
CONCLUSION
The overall difference between first-trimester screening based on fetal NT and maternal serum biochemistry with and without maternal age is about 3%. In screening with a fixed cut-off, the maternal age-related risk keeps the false-positive rate low in younger women and increases the detection rate in older women.
To examine the impact of the maternal age-related risk in first-trimester combined screening for trisomy 21.
METHODS
Prospective assessment of risk for trisomy 21 by a combination of maternal age, fetal NT thickness and maternal serum PAPP-A and free textgreekb-hCG at 11+0 to 13+6 weeks of gestation between April 2002 and February 2007. Screening for trisomy 21 by patient-specific risks based on the maternal and gestational age-related risk multiplied by a likelihood ratio for NT and for maternal serum biochemistry were compared with a screening policy that is only based on the combined likelihood ratio for fetal NT and maternal serum biochemistry.
RESULTS
The study population consisted of 38,603 euploid pregnancies and 109 fetuses with trisomy 21. In screening for trisomy 21 by fetal NT and maternal serum biochemistry in combination with and without maternal age with a fixed false-positive rate of 3%, the detection rate was 82.6 and 79.8%, respectively. In the group of women with a maternal age of less than 30 years and between 30 and 35 years, there was no difference in the detection rate. For women with a maternal age of 35 years or older, the detection rate increased from 77.1% without maternal age to 94.3% with maternal age, respectively.
CONCLUSION
The overall difference between first-trimester screening based on fetal NT and maternal serum biochemistry with and without maternal age is about 3%. In screening with a fixed cut-off, the maternal age-related risk keeps the false-positive rate low in younger women and increases the detection rate in older women.
Schmidt, T; Dirks, J; Enders, Martin; Sester, U; Gärtner, B; Sester, M
Pandemic H1N1 influenza vaccination induces T-cell immunity with notable cross-reactivity agains seasonal influenza: Abstract Proceedings Article
In: 21st Annual Meeting of the Society for Virology, S. 644, 2011.
@inproceedings{Schmidt.2011,
title = {Pandemic H1N1 influenza vaccination induces T-cell immunity with notable cross-reactivity agains seasonal influenza: Abstract},
author = {T Schmidt and J Dirks and Martin Enders and U Sester and B G\"{a}rtner and M Sester},
year = {2011},
date = {2011-01-01},
booktitle = {21st Annual Meeting of the Society for Virology},
pages = {644},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
Terletskaia-Ladwig, Elena; Enders, Gisela; Meier, S; Dietz, K; Enders, Martin
In: Journal of virological methods, Bd. 178, Nr. 1-2, S. 124–128, 2011, ISSN: 0166-0934.
@article{TerletskaiaLadwig.2011b,
title = {Development and evaluation of an automatable focus reduction neutralisation test for the detection of measles virus antibodies using imaging analysis},
author = {Elena Terletskaia-Ladwig and Gisela Enders and S Meier and K Dietz and Martin Enders},
doi = {10.1016/j.jviromet.2011.08.026},
issn = {0166-0934},
year = {2011},
date = {2011-01-01},
journal = {Journal of virological methods},
volume = {178},
number = {1-2},
pages = {124--128},
abstract = {A plaque reduction neutralisation test (PRNT) is still regarded as the gold standard for the investigation of anti-measles immunity. In this study, an alternative simplified automatable focus reduction neutralisation test (AFRNT) based on the classical PRNT was developed. The AFRNT uses the conventional Edmonston strain of measles, immunoperoxidase staining with monoclonal antibodies, and automated plaque counts performed with AID ViruSpot software. The assay is performed in 96-well plates, requires 2 days, and is fully automatable. The AFRNT was evaluated in comparison with PRNT and Enzygnost anti-measles enzyme immunoassay (EIA). A total of 130 samples, which included two available WHO international anti-measles standards, sera from 90 patients, and 38 different lots of immunoglobulin products, were tested. Overall, good agreement was observed between EIA and both neutralisation tests; however, the EIA values for the immunoglobulin products and international standards were slightly but significantly higher than those of the neutralisation tests. The Bland-Altman analysis showed excellent agreement between AFRNT and PRNT. AFRNT is a fully automatable high-throughput neutralisation assay, which can be performed with measles and other types of viruses, including wild-type strains. It is perfectly suited for epidemiological and vaccine studies},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A plaque reduction neutralisation test (PRNT) is still regarded as the gold standard for the investigation of anti-measles immunity. In this study, an alternative simplified automatable focus reduction neutralisation test (AFRNT) based on the classical PRNT was developed. The AFRNT uses the conventional Edmonston strain of measles, immunoperoxidase staining with monoclonal antibodies, and automated plaque counts performed with AID ViruSpot software. The assay is performed in 96-well plates, requires 2 days, and is fully automatable. The AFRNT was evaluated in comparison with PRNT and Enzygnost anti-measles enzyme immunoassay (EIA). A total of 130 samples, which included two available WHO international anti-measles standards, sera from 90 patients, and 38 different lots of immunoglobulin products, were tested. Overall, good agreement was observed between EIA and both neutralisation tests; however, the EIA values for the immunoglobulin products and international standards were slightly but significantly higher than those of the neutralisation tests. The Bland-Altman analysis showed excellent agreement between AFRNT and PRNT. AFRNT is a fully automatable high-throughput neutralisation assay, which can be performed with measles and other types of viruses, including wild-type strains. It is perfectly suited for epidemiological and vaccine studies
Hofmann, J; Ettinger, J; Rainer, U; Popugaeva, E; Klempa, B; Enders, Martin; Meier, M; Führer, A; Krüger, D
Human hantavirus disease due to infection by Dobrava-Belgrade virus in northern germany - the first comprehensive study: Abstract Proceedings Article
In: 21st Annual Meeting of the Society for Virology, S. 321, 2011.
@inproceedings{Hofmann.2011,
title = {Human hantavirus disease due to infection by Dobrava-Belgrade virus in northern germany - the first comprehensive study: Abstract},
author = {J Hofmann and J Ettinger and U Rainer and E Popugaeva and B Klempa and Martin Enders and M Meier and A F\"{u}hrer and D Kr\"{u}ger},
year = {2011},
date = {2011-01-01},
booktitle = {21st Annual Meeting of the Society for Virology},
pages = {321},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
Ettinger, J; Enders, Martin; Ulrich, R; Essbauer, S; Klempa, B; Krüger, D; Hofmann, J
Molecular phylogenetic clades of puumalavirus from the 2010 outbreak in germany: Abstract Proceedings Article
In: 21st Annual Meeting of the Society for Virology, S. 597, 2011.
@inproceedings{Ettinger.2011,
title = {Molecular phylogenetic clades of puumalavirus from the 2010 outbreak in germany: Abstract},
author = {J Ettinger and Martin Enders and R Ulrich and S Essbauer and B Klempa and D Kr\"{u}ger and J Hofmann},
year = {2011},
date = {2011-01-01},
booktitle = {21st Annual Meeting of the Society for Virology},
pages = {597},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
2010
Sallmén, M; Riipinen, A; Söderlund-Venermo, M; Lindbohm, M; Hedman, K; Karikoski, R; Nuutila, M; Taskinen, H; Enders, M
In: EPICOH Annual Meeting, Bd. 60, S. A2, 2010.
@incollection{Sallmen.2010,
title = {Parvovirus B19 infection and intrauterine foetal death: conflicting findings in studies of different types: Abstract A006: Abstract},
author = {M Sallm\'{e}n and A Riipinen and M S\"{o}derlund-Venermo and M Lindbohm and K Hedman and R Karikoski and M Nuutila and H Taskinen and M Enders},
doi = {10.1136/oem.2010.60608.6},
year = {2010},
date = {2010-01-01},
booktitle = {EPICOH Annual Meeting},
volume = {60},
pages = {A2},
keywords = {},
pubstate = {published},
tppubtype = {incollection}
}
Eggers, Maren; Terletskaia-Ladwig, Elena; Rabenau, Holger F; Doerr, Hans W; Diedrich, Sabine; Enders, Gisela; Enders, Martin
In: BMC infectious diseases, Bd. 10, Nr. 1, S. 115, 2010, ISSN: 1471-2334.
@article{Eggers.2010,
title = {Immunity status of adults and children against poliomyelitis virus type 1 strains CHAT and Sabin (LSc-2ab) in Germany},
author = {Maren Eggers and Elena Terletskaia-Ladwig and Holger F Rabenau and Hans W Doerr and Sabine Diedrich and Gisela Enders and Martin Enders},
doi = {10.1186/1471-2334-10-347},
issn = {1471-2334},
year = {2010},
date = {2010-01-01},
journal = {BMC infectious diseases},
volume = {10},
number = {1},
pages = {115},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Enders, M; Reiter-Owona, I; Knotek, F; Rilling, V; Krczal, D; Enders, G
Seroepidemiology of Toxoplasma gondii infection in pregnant women from Southern and North-Western Germany and bone-marrow donors: Abstract Buchabschnitt
In: für Chemotherapie e.V., Paul-Ehrlich-Gesellschaft (Hrsg.): 22. Jahrestagung der Paul-Ehrlich-Gesellschaft für Chemotherapie e.V. Abstracts - Reihenfolge nach Programm, S. 166, 2010.
@incollection{Enders.2010,
title = {Seroepidemiology of Toxoplasma gondii infection in pregnant women from Southern and North-Western Germany and bone-marrow donors: Abstract},
author = {M Enders and I Reiter-Owona and F Knotek and V Rilling and D Krczal and G Enders},
editor = {{Paul-Ehrlich-Gesellschaft f\"{u}r Chemotherapie e.V.},
year = {2010},
date = {2010-01-01},
booktitle = {22. Jahrestagung der Paul-Ehrlich-Gesellschaft f\"{u}r Chemotherapie e.V. Abstracts - Reihenfolge nach Programm},
pages = {166},
keywords = {},
pubstate = {published},
tppubtype = {incollection}
}